{"id":6195,"date":"2023-06-12T14:47:48","date_gmt":"2023-06-12T12:47:48","guid":{"rendered":"https:\/\/veterinarska-stanica-journal.hr\/?post_type=article&p=6195"},"modified":"2023-07-12T11:39:14","modified_gmt":"2023-07-12T09:39:14","slug":"prevalence-and-infestation-degree-of-yeast-in-canine-otitis-externa","status":"publish","type":"article","link":"https:\/\/journal.h3s.org\/?article=prevalence-and-infestation-degree-of-yeast-in-canine-otitis-externa","title":{"rendered":"Prevalence and infestation degree of yeast in canine otitis externa<\/em>"},"content":{"rendered":"

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N. Te\u0161in<\/strong>, I. Stan\u010di\u0107<\/strong>, Z. Kana\u010dki<\/strong>, J. Spasojevi\u0107<\/strong>, Z. Ru\u017ei\u0107<\/strong>, I. Gali\u0107<\/strong> and Z. Kova\u010devi\u0107<\/strong>*<\/p>\n

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Nade\u017eda TE\u0160IN<\/strong>, DVM, Junior Researcher, Ivan STAN\u010cI\u0106<\/strong>, DVM, PhD, Full Professor, Zdenko KANA\u010cKI<\/strong>, DVM, PhD, Full Professor, Jovan SPASOJEVI\u0106<\/strong>, DVM, PhD, Assistant Professor, Zoran RU\u017dI\u0106<\/strong>, DVM, PhD, Assistant Professor, Ivan GALI\u0106<\/strong>, DVM, PhD, Teaching Assistant, Zorana KOVA\u010cEVI\u0106<\/strong>*, PhD, Associate Professor (Corresponding author, e-mail: zorana.kovacevic@polj.edu.rs), Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia<\/div>\n
\"\"<\/a>https:\/\/doi.org\/10.46419\/vs.54.6.7<\/a><\/div>\n<\/p>\n

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Abstract<\/a>Introduction<\/a>Materials and methods<\/a>Results and discussion<\/a>Conclusion<\/a>References<\/a>Sa\u017eetak<\/a><\/span><\/div>\n<\/div>\n

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Abstract<\/h2>\n
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Otitis externa<\/em> is a persistent and recurrent problem, and the resistance of the involved pathogens to conventional drugs has made this a serious issue in veterinary medicine. This is an inflammation of the external ear canal in dogs that can be caused by bacteria or yeast.
\nOften, these two causative agents are isolated together in combination. This study aimed to determine the prevalence of yeast and the degree of infestation of the isolated pathogens in canine otitis externa<\/em> at veterinary clinics for small pets at the Department of Veterinary Medicine, University of Novi Sad, Serbia.
\nSamples were taken from 60 dogs with clinical symptoms of otitis externa<\/em> using a sterile swab and sent to laboratory for further mycological and bacteriological examination. In order to isolate the bacteria and yeast, conventional methods were used under aerobic conditions.
\nOf the total samples, 53 (88.33%) were positive for at least one of the causative agents of otitis externa<\/em>. Yeasts were isolated from 36 (52.94%) samples. The study showed that in comparing mixed and independent types of infection, in most cases 15\/23 (65.22%) the degree of infestation with yeast was highest when the infection was mixed with bacteria. In relation to dog breeds, their ear forms and type of infection, more yeast samples (either as solo or mixed infection) were isolated from dogs with pendulous ears. It has been confirmed that Malassezia pachydermatis<\/em> is the most common yeast pathogen of otitis externa<\/em> in dogs, compared to other species of this genus, and it occurs in a higher degree of infestation in mixed infections than in solo infections. Dog breeds with pendulous ear form are more predisposed to yeast otitis externa<\/em> than erect eared dog breeds.<\/p>\n

Key words:<\/strong> otitis externa; Malassezia spp.; dog breed<\/em><\/p><\/blockquote>\n

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Introduction<\/h2>\n
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Otitis externa<\/em> of dogs is an acute or chronic inflammation of the external ear canal including the ear pinna and is one of the most common reasons why owners take their dogs to the vet (Woodwars, 2022). Usually, bacteria and yeast are considered to be secondary causes of this disease (Lyskova et al<\/em>., 2007). Moreover, small number of these potentially pathogenic microorganisms can be found normally in the external ear canal, but when an environmental or primary factors disturb the normal conditions in the ear, the potential for their virulent action is increased (De Martino et al<\/em>., 2016).<\/p>\n

Additionally, bacteria are frequently present in ear affected by otitis externa<\/em> and the most commonly isolated species from dog ear canals are Staphylococcus<\/em> spp., Streptococcus<\/em> spp., Pseudomonas<\/em> spp., Escherichia coli<\/em> and other Enterobacteriaceae<\/em> (Zamankhan Malayeri et al<\/em>., 2010; Ebani et al<\/em>., 2017; Bajwa, 2019). Furthermore, bacteria and yeast are often present together in combination (Little, 1996).<\/p>\n

One of the most frequent agents in canine otitis externa<\/em> is Malassezia<\/em> yeast, with a prevalence as a sole causative agent between 8% and 26% (Rougier et al<\/em>., 2005; King et al<\/em>., 2018). Furthermore, the genus Malassezia<\/em> includes seven species: M. pachydermatis<\/em>, M. globosa<\/em>, M. furfur<\/em>, M. slooffiae<\/em>, M. sympodialis<\/em>, M. obtusa<\/em> and M. restricta<\/em>. All species except M. pachydermatis are lipid-dependent for growth (Gueho et al<\/em>., 1996). Furthermore, M. pachydermatis<\/em> is the most common isolated yeast from affected ears and in stained smears appears like bottle- or \u2018bowling pin\u2019- shaped cells (Weidman, 1925) and is often attached to exfoliated epithelial cells (Kowalski, 1988).<\/p>\n

In dogs, persistent or recurrent Malassezia<\/em> dermatitis is usually associated with failure to identify and correct predisposing or perpetuating factors (Guillot and Bond, 2020). Although skin changes may be more prevalent in a certain area, larger parts of the body are usually affected, such as the limbs, underside, ears and face. In the case of otitis occurring at the same time with dermatitis, there are changes in the external ear canal that imply glandular hyperplasia and dilation, epithelial hyperplasia and hyperkeratosis which leads to lichenification, scaling and increasing yellow or brownish cerumen production (Bond et al<\/em>., 2010; Bajwa, 2019).<\/p>\n

In case of otitis externa<\/em> treatment caused by yeast, despite antifungal therapy, many cases do not respond and remain unresolved for long periods, until they are resolved spontaneously or became a more serious problem (Bernardo et al<\/em>., 1998). However, the evidence that reduced susceptibility of M. pachydermatis<\/em> to commonly used antifungal drugs may develop under both field and laboratory conditions highlights the need for surveillance and vigilance for the emergence of clinically-relevant resistance (Guillot and Bond, 2020).<\/p>\n

Although M. pachydermatis<\/em> is mainly associated with animals, transmission and infections to newborn babies and immunocompromised people is known (Chang et al<\/em>., 1988; Mani and Maguire, 2009). Interestingly, it could represent a One Health<\/em> issue since the transference from animals to human is determined.<\/p>\n

Hence, the aim of the present study was to determine the prevalence of the yeast in canine otitis externa<\/em> at veterinary clinics for small pets at the Department of Veterinary Medicine, University of Novi Sad in Serbia, and the degree of infestation of the isolated pathogens.<\/p>\n

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Materials and methods<\/h2>\n
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Sample collection<\/h3>\n

The research was conducted at the Veterinary Clinic of the Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Serbia over a two-year period (October 2020 to October 2022). In total, 60 samples from the external ear canal from dogs with clinical symptoms of otitis externa<\/em> were examined mycologically and bacteriologically. Dogs were of different breeds, age and gender.
\nFurthermore, clinical symptoms of otitis externa<\/em> were determined in at least one ear, established by the anamnesis from the owner and by physical and otoscopic examination of the dogs. Samples were collected with sterile cotton-tipped swabs, transferred to a tube with transport media (EUROTUBO\u00ae<\/sup>) and maintained at 4\u00b0C until transport the same day to the Vetlab veterinary laboratory in Belgrade for further testing.<\/p>\n

Isolation and identification of pathogens<\/h3>\n

To isolate bacteria, conventional microbiological methods were used under aerobic conditions (Columbia agar, Biomerieux, France), commercial biochemical tests were used for identification, and antibiotic susceptibility testing was performed by disc diffusion method according to European Society of Clinical Microbiology and Infectious Diseases (EUCAST) guidelines.<\/p>\n

To isolate yeast, the conventional method was used under aerobic conditions (SDA agar, Promedia, Serbia).
\nConfirmation of the identification and concentration of Malassezia pachydermatis<\/em> in otitis externa<\/em> is additionally performed with microscopy. The concentration, precisely the degree of infestation is estimated based on the average number of yeast cells per epithelial cell on the cytological preparation (Figure 1).<\/p>\n

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Figure 1<\/strong>. Microscopic morphology of Malassezia pachydermatis<\/em>.<\/figcaption><\/figure>\n

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Results and discussion<\/h2>\n
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Bacteriological and mycological examination (Figure 2) found that of the 60 samples, 53 (88.33%) were positive for at least one of the infectious secondary causative agents of otitis externa<\/em>.<\/p>\n

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Figure 2<\/strong>. Prevalence of otitis externa<\/em> causing pathogens in the collected samples.<\/figcaption><\/figure>\n

In the remaining 7 samples (11.67%), neither bacteria nor yeast were found. Furthermore, 23 (38.33%) samples were with a mixed infection, bacteria and yeast, 19 (31.67%) samples were positive for G+ bacteria, while the remaining samples 4 (6.67%) were mixed with G- bacteria.
\nThese results are similar to those obtained by Aalb\u00e6k et al<\/em>. (2010), where 23 of 55 samples were mixed cultures. Also, Saridomichelakis et al<\/em>. (2007) reported that at least one of these organisms was present in 93 of 100 cases, whereas the coexistence of two or more was observed in 33 of 100 dogs. Contrary to our findings, other research results have shown that mixed infections occurred in more than half of otitis externa<\/em> affected dogs, where the combination of Staphylococcus intermedius<\/em> and Malassezia pachydermatis<\/em> was most common (Oliveira et al<\/em>. 2008; Petrov et al<\/em>., 2013). Moreover, Kiss et al<\/em>. (1996) stated that this phenomenon might be related to the capability of these two microorganisms to utilise each other\u2019s metabolic products, i.e., the proteolytic products of Malassezia<\/em> or the nicotinic acid produced by staphylococci. The results presented here show that samples where only bacteria were isolated were present in 17 (28.33%) cases, of which 12 (20%) were with G+ and 5 (7.35%) with G- bacteria.<\/p>\n

Perry et al<\/em>. (2017) established that yeasts were associated with the majority of infections (82.0%), either as sole or mixed infections. In the present study, yeasts were isolated from 36 (52.94%) samples, though only solely yeast infection in 13 (19.12%) cases. Among the yeast pathogens, the most common was Malassezia<\/em> yeast species, as the only type isolated. In a Hungarian study conducted on 210 isolates, it is determined that the most common isolate was the yeast M. pachydermatis<\/em>, which was isolated in pure culture in 43.30% of cases and in association with different bacteria in 33.00% of cases (Kiss et al<\/em>., 1997).<\/p>\n

The type of infection and degree of infestation of Malassezia pachydermatis<\/em> in dogs with diagnosed otitis externa<\/em> are shown in Table 1.<\/p>\n

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Table 1<\/strong>. Type of infection and degree of infestation of Malassezia pachydermatis<\/em> in dogs with diagnosed otitis externa<\/em>.
*PUG-Pug, LR-Labrador Retriever, GSD-German Shepherd Dog, SHIH-Shih Tzu, POM-Pomeranian, MLT-Maltese, MIXED-Mixed Breed, AK-Akita, POODL-Poodle (Toy & Miniature), BT-Boston Terrier, DH-Dachshund (Miniature, Standard), DOGO-Dogo Argentino, SBT-Staffordshire Bull Terrier, BF-Bichon Frise, LAGR-Lagotto Romagnolo, APBT-American Pit Bull Terrier, BGL-Beagle, PULI-Puli, HWV-Hungarian Wire-Haired Vizsla, WT-Welsh Terrier, BOL-Bolognese, WHWT-West Highland White Terrier, GR-Golden Retriever<\/figcaption><\/figure>\n

In cases where the yeast appeared independently, a first-degree infection was present in most cases 8\/13 (61.54%), second degree in 3\/13 (23.08%), while third-degree infection cases occurred the least with 2 of 13 cases (15.38%). These results show that the most dominant in independent otitis externa<\/em> infection was a first-degree infestation. Regarding the comparison between mixed and independent types of infection, in most cases 15\/23 (65.22%) the degree of infestation with yeast was highest when the infection was mixed with bacteria. The reason might be that mixed infection could provoke otitis externa<\/em> infection to be more serious with more complicated clinical symptoms.
\nAlthough most owners and veterinarians recognize ear infections, the ongoing inflammation is often missed. This can lead to chronic infection or otitis media that is more difficult to treat and takes longer to resolve (Nuttall, 2016).
\nMoreover, first-degree yeast infections were present in 6\/23 (26.09%) cases and only 2\/23 (8.70%) were present with second-degree yeast infections.
\nThe relation between dog breeds, their ear forms and type of infection are shown in Table 2.<\/p>\n

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Table 2<\/strong>. Type of infection in dog breeds in relation to ear form.
*PUG-Pug, LR-Labrador Retriever, GSD-German Shepherd Dog, SHIH-Shih Tzu, POM-Pomeranian, MLT-Maltese, MIXED-Mixed Breed, AK-Akita, POODL-Poodle (Toy & Miniature), BT-Boston Terrier, DH-Dachshund (Miniature, Standard), DOGO-Dogo Argentino, SBT-Staffordshire Bull Terrier, BF-Bichon Frise, LAGR-Lagotto Romagnolo, APBT-American Pit Bull Terrier, BGL-Beagle, PULI-Puli, HWV-Hungarian Wire-Haired Vizsla, WT-Welsh Terrier, BOL-Bolognese, WHWT-West Highland White Terrier, GR-Golden Retriever
*P-Pendulous ears, E-Erect ears<\/figcaption><\/figure>\n

Isolates from dogs with pendulous ears were present in 25 of 36 samples (69.44%), while 11\/36 (30.56%) were isolated from erect eared dogs. In the pendulous group, yeast otitis externa<\/em> occurred most often in Maltese 4\/36 (11.11%), Mixed breed 4\/36 (11.11%) and Pug 3\/36 (8.33%), while in the erect ear group German Shepherd Dog and American Pit Bull Terrier, both with 2 of 36 (5.55%) cases, had a larger number of otitis cases than other dog breeds. Moreover, this study also shows that the proportion of mixed and yeast infections was not significantly different among dog breeds in relation to ear form, and mixed infection were dominant in both groups. In pendulous ear breeds, mixed infection was present in more samples 15\/25 (60%) than only yeast infection 10\/25 (40%). The same is the case with erect ear breeds where mixed infection was present in almost all samples 8\/11(72.73%) while the remaining 3\/11 (27.27%) samples was with only yeast infection.<\/p>\n

Yeast otitis externa<\/em> has been recognized as a very common cause of dermatitis in dogs (Larsson et al<\/em>., 1988; Bond et al<\/em>., 1995), though this is not equally prevalent in different dog breeds (Perry et al<\/em>., 2017). Factors that increase the risk of this disease include form of the ear canal and pinna, hair growth, stenotic ears, increased cerumen production, ear trauma and increase in humidity (Paterson, 2016; Bajwa, 2019). Some dogs may develop an allergic reaction to Malassezia<\/em> spp., which can consequently lead to discomfort and pruritus (Bajwa, 2019) and, when it not correctly treated, the condition can turn into otitis media (Boone et al<\/em>., 2021). Furthermore, Malassezia<\/em> yeast and bacteria such as Staphylococcus<\/em> and Pseudomonas<\/em> are common perpetuating factors that do not initiate inflammation but lead to exacerbation of the inflammatory process and maintain ear disease even if the primary factor has been identified and corrected. Moreover, if infection travels to the tympanic bulla, the presence of this infection in the middle ear can also act as a perpetuating factor, leading to recurrent external ear infections. Perpetuating factors are often the main reason for treatment failure in dogs affected by recurrent otitis externa<\/em> (Bajwa, 2019).<\/p>\n

Keeping in mind the routine sensitivity and in terms of absence of standard methods suitable for the Malassezia<\/em> genus, diagnostic testing in veterinary practice should preferably be based on cytological than on culture methods (Guillot and Bond, 2020). In addition, topical antimicrobial therapy is preferred to systemic treatment in the case of otitis externa<\/em>, but frequently it is not successful because of the resistance of bacteria and yeast agents to conventional drugs (Ebani et al<\/em>., 2023). Also, such an application is often not dosed correctly (Bernardo et al<\/em>., 1998). Moreover, effective therapy, together with the correction of predisposing factors and concurrent diseases, is mandatory to prevent relapses (Guillot and Bond, 2020). That is the reason why the agreed reference methods to assess antifungal susceptibility of M. pachydermatis<\/em> are required to assist veterinary practitioner in the management of chronic cases (Guillot and Bond, 2020).<\/p>\n

In the prevention of otitis externa<\/em> and its complications that may lead to chronic otitis, hearing loss, otitis media, and end-stage ear disease, there are few effective preventive measures. Otic examination of all patients presented for a physical examination can assist in early detection of mild and early cases of otitis. Moreover, when dogs are presented with early ear disease, thorough client education and detailed diagnostic workup, including frequent follow-up examinations, can help prevent development of complications (Bajwa, 2019).<\/p>\n

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Conclusion<\/h2>\n
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In conclusion, this study aimed to determine the prevalence and degree of infestation of yeast in otitis externa<\/em> in dogs. <\/p>\n

The results showed that the only yeast species isolated was Malassezia pachydermatis<\/em>. Isolation of higher levels of Malassezia pachydermatis<\/em> appears to play a significant role in canine otitis externa<\/em>, both in independent and mixed otitis externa<\/em> infections. As bacteria become more and more resistant to antibiotics, which is currently a global problem, fungal infections should not be disregard and therefore further research is needed for in vitro<\/em> testing of antifungal sensitivity of the isolated pathogens. The application of molecular typing methods will allow for recognition of more cases in the future in light of the One Health<\/em> concept, since awareness of the potential for the transfer of Malassezia<\/em> spp. to human patients is increased.<\/p>\n

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\nReferences<\/strong> [… show]<\/span><\/a><\/span><\/p>\n

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1.\tAALB\u00c6K, B., D. A. BEMIS, M. SCHJ\u00c6RFF, S. A. KANIA, L. A. FRANK and L. GUARDABASSI (2010): Coryneform bacteria associated with canine otitis externa. Vet. Microbiol. 145, 292-298. 10.1016\/j.vetmic.2010.03.032
\n2.\tBAJWA, J. (2019): Canine otitis externa-Treatment and complications. Can. Vet. J. 60, 97-99.
\n3.\tBERNARDO, F. M., H. M. MARTINS and M. L. MARTINS (1998): A survey of mycotic otitis externa of dogs in Lisbon. Rev. Iberoam. Micol. 15, 163-165.
\n4.\tBOND, R., J. GUILLOT and F. J. CABA\u00d1ES (2010): Malassezia Yeasts in Animal Disease. In: Boekhout, T., P. Mayser, E. Gu\u00e9ho-Kellermann and A. Velegraki: Malassezia and the Skin. Springer, Berlin, Heidelberg (271-299).
\n5.\tBOND, R., J. F. ROSE, J. W. ELLIS and D. H. LLOYD (1995): Comparison of two shampoos for treatment of Malassezia pachydermatis-associated seborrhoeic dermatitis in Basset Hounds. J. Small. Anim. Prac. 36, 99-104. 10.1111\/j.1748-5827.1995.tb02840.x BOONE, J. M., R. BOND, A. LOEFFER, E. A.
\n6.\tFERGUSON and A. Hendricks (2021): Malassezia otitis unresponsive to primary care: Outcome in 59 dogs. Vet. Dermatol. 32, 441. 10.1111\/vde.12995
\n7.\tCHANG, H. J., H. L. MILLER, N. WATKINS, M. J. ARDUINO, D. A. ASHFORD, G. MIDGLEY et al. (1998): An epidemic of Malassezia pachydermatis in an intensive care nursery associated with colonization of health care workers\u2019 pet dogs. N. Engl. J. Med. 338, 706-711. 10.1056\/ NEJM199803123381102
\n8.\tDE MARTINO, L., F. P. NOCERA, K. MALLARDO, S. NIZZA, E. MASTURZO, F. FIORITO et al. (2016): An update on microbiological causes of canine otitis externa in Campania Region, Italy. Asian Pac. J. Trop. Biomed. 6, 384-389. 10.1016\/j.apjtb.2015.11.012
\n9.\tEBANI, V. V., S. NARDONI, F. BERTELLONI, B. NAJAR, L. PISTELLI and F. MANCIANTI (2017): Antibacterial and Antifungal Activity of Essential Oils against Pathogens Responsible for Otitis Externa in Dogs and Cats. Medicines 4, 21. 10.3390\/ medicines4020021
\n10.\tEBANI, V. V., Y. PIERACCI, G. CAGNOLI, F. BERTELLONI, C. MUNAF\u00d2, S. NARDONI and F. MANCIANTI (2023): In Vitro Antimicrobial Activity of Thymus vulgaris, Origanum vulgare, Satureja montana and Their Mixture against Clinical Isolates Responsible for Canine Otitis Externa. Vet. Sci. 10, 30. 10.3390\/vetsci10010030
\n11.\tGU\u00c9HO, E., G. MIDGLEY and J. GUILLOT (1996): The genus Malassezia with description of four new species. Antonie van Leeuwenhoek 69, 337-355. 10.1007\/BF00399623
\n12.\tGUILLOT, J. and R. BOND (2020): Malassezia yeasts in veterinary dermatology: an updated overview. Front. Cell. Infect. Microbiol. 79. 10.3389\/ fcimb.2020.00079
\n13.\tKING, S.B., K. P. DOUCETTE, W. SEEWALD and S. L. FORSTER (2018): A randomized, controlled, single-blinded, multicenter evaluation of the efficacy and safety of a once weekly two dose otic gel containing florfenicol, terbinafine and betamethasone administered for the treatment of canine otitis externa. BMC Vet. Res. 14, 307. 10.1186\/s12917-018-1627-5
\n14.\tKISS, G., S. Z. RADVANYI and G. SZIGETI (1996): Characteristics of Malassezia pachydermatis strains isolated from canine otitis externa. Mycoses. 39, 313-321. 10.1111\/j.1439-0507.1996.tb00146.x
\n15.\tKISS, G., S. Z. RADVANYI and G. SZIGETI (1997): New combination for the therapy of canine otitis externa I Microbiology of otitis externa. J. Small. Anim. Pract. 38, 51-56. 10.1111\/j.1748-5827.1997.tb02987.x
\n16.\tKOWALSKI, J. J. (1988): The Microbial Environment of the Ear Canal in Health and Disease. Vet. Clin. N. Am. – Small Anim. Pract. 18, 743-754. 10.1016\/s0195- 5616(88)50077-3
\n17.\tLARSSON, C. E., M. H. M. A. LARSSON, R. C. D AMARAL, C. R. PAULA, M. K. HAGIWARA and W. R. FERNANDES (1988): Dermatitis in dogs caused by Malassezia (Pityrosporum) pachydermatis. Ars Vet. 4, 63-68.
\n18.\tLITTLE, C. (1996): Medical treatment of otitis externa in the dog and cat. In Pract. 18, 66-71. 10.1136\/inpract.18.2.66
\n19.\tLYSKOVA, P., M. VYDRZALOVA and J. MAZUROVA (2007): Identification and Antimicrobial Susceptibility of Bacteria and Yeasts Isolated from Healty Dogs and Dogs with Otitis Externa. J. Vet. Med. 54, 559-563. 10.1111\/j.1439- 0442.2007.00996.x
\n20.\tMANI, I. and J. MAGUIRE (2009): Small Animal Zoonoses and Immunocompromised Pet Owners. Top. Companion Anim. Med. 24, 164-174. 10.1053\/j.tcam.2009.07.002
\n21.\tNUTTALL, T. (2016): Successful management of otitis externa. In Pract. 38, 17-21. 10.1136\/inp.i1951
\n22.\tOLIVEIRA, L. C., C. A. LEITE, R. S. BRILHANTE and C. B. CARVALHO (2008): Comparative study of the microbial profile from bilateral canine otitis externa. Can. Vet. J. 49, 785-788.
\n23.\tPATERSON, S. (2016): Discovering the causes of otitis externa. In Pract. 38, 7-11. 10.1136\/inp.i470
\n24.\tPERRY, L. R., B. MACLENNAN, R. KORVEN and T. A. RAWLINGS (2017): Epidemiological study of dogs with otitis externa in Cape Breton, Nova Scotia. Can. Vet. J. 58, 168-174.
\n25.\tPETROV, V., G. MIHAYLOV, I. TSACHEV, G. ZHELEV, P. MARUTSOV and K. KOEV (2013): Otitis externa in dogs: microbiology and antimicrobial susceptibility. Revue M\u00e9d. V\u00e9t. 164, 18-22.
\n26.\tROUGIER, S., D. BORELL, S. PHEULPIN, F. WOEHRL\u00c8 and B. BOISRAM\u00c9 (2005): A comparative study of two antimicrobial\/anti-inflammatory formulations in the treatment of canine otitis externa. Vet. Dermatol. 16, 299-307. 10.1111\/j.1365-3164.2005.00465.x
\n27.\tSARIDOMICHELAKIS, M. N., R. FARMAKI, L. S. LEONTIDES and A. F. KOUTINAS (2007): Aetiology of canine otitis externa: a retrospective study of 100 cases. Vet. Dermatol. 18, 341-347. 10.1111\/j.1365-3164.2007.00619.x
\n28.\tWEIDMAN, F. D. (1925): Exfoliative dermatitis in the Indian rhinoceros (Rhinoceros unicornis), with description of a new species: Pityrosporum pachydermatis. In: Fox, H. (ed.), Rep Lab Museum Comp Pathol Zoo Soc Philadelphia, Philadelphia (36-43).
\n29.\tWOODWARS, M. (2022): Otitis externa in animals. In MERCK Veterinary Manual. Available via https:\/\/www.merckvetmanual.com\/ear-disorders\/ otitis-externa\/otitis-externa-in-animals (Accessed January 2023.)
\n30.\tZAMANKHAN MALAYERI, H., S. JAMSHIDI and T. ZAHRAEI SALEHI (2010): Identification and antimicrobial susceptibility patterns of bacteria causing otitis externa in dogs. Vet. Res. Commun. 34, 435-444. 10.1007\/s11259-010-9417-y
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Prevalencija i stupanj infestacije gljivica kod upale vanjskog u\u0161nog kanala u pasa<\/h2>\n
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Nade\u017eda TE\u0160IN<\/strong>, dr. med. vet., istra\u017eiva\u010d pripravnik, dr. sc. Ivan STAN\u010cI\u0106<\/strong>, dr. med. vet., redoviti profesor, dr. sc. Zdenko KANA\u010cKI<\/strong>, dr. med. vet., redoviti profesor, dr. sc. Jovan SPASOJEVI\u0106<\/strong>, dr. med. vet., docent, dr. sc. Zoran RU\u017dI\u0106<\/strong>, dr. med. vet., docent, dr. sc. Ivan GALI\u0106<\/strong>, dr. med. vet., asistent, dr. sc. Zorana KOVA\u010cEVI\u0106<\/strong>, izvanredna profesorica, Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu, Novi Sad, Srbija<\/div>\n
\n

Kao perzistentan i rekurentan problem, zbog prisustva rezistencije patogenih uzro\u010dnika na konvencionalne lijekove upala vanjskog u\u0161nog kanala predstavlja ozbiljan problem u veterinarskoj medicini. Upala vanjskog u\u0161nog kanala u pasa mo\u017ee biti prouzro\u010dena bakterijama i gljivicama. Ova se dva uzro\u010dnika \u010desto mogu izolirati zajedno, u kombinaciji. Predmet je ovog istra\u017eivanja bio utvr\u0111ivanje prevalencije gljivica i stupnja infestacije izoliranih patogena kod upale vanjskog u\u0161nog kanala u pasa u Veterinarskoj klinici za male \u017eivotinje Departmana za veterinarsku medicinu u Novom Sadu, Srbija.
\nUzorci su uzeti od 60 pasa s klini\u010dkim simptomima upale vanjskog u\u0161nog kanala pomo\u0107u sterilnog brisa, a poslani su u Laboratorij na daljnje mikolo\u0161ko i bakteriolo\u0161ko ispitivanje. Da bi se izolirale bakterije i gljivice, uporabljene su konvencionalne metode u aerobnim uvjetima. Od ukupnog broja uzoraka, 53 (88,33 %) su bila pozitivna na prisustvo najmanje jednog od uzro\u010dnika upale vanjskog u\u0161nog kanala. Gljivice su izolirane iz ukupno 36 (52,94 %) uzoraka. Istra\u017eivanje je pokazalo da je u slu\u010daju usporedbe izme\u0111u mije\u0161anih i samostalnih tipova infekcije u najve\u0107em broju slu\u010dajeva 15\/23 (65,22 %) stupanj infestacije gljivicama bio najvi\u0161i kada je infekcija bila pomije\u0161ana s bakterijama. U odnosu na pasmine pasa, njihove tipove u\u0161iju i vrstu infekcije, utvr\u0111eno je da je vi\u0161e uzoraka s gljivicama bilo kao samostalna ili mije\u0161ana infekcija izolirano u pasa s vise\u0107im u\u0161ima. Potvr\u0111eno je da je Malassezia pachydermatis<\/em> naj\u010de\u0161\u0107i gljivi\u010dni patogen upale vanjskog u\u0161nog kanala pasa, u usporedbi s drugim vrstama ovoga roda. Kod mje\u0161ovite infekcije javlja se u ve\u0107em stupnju infestacije nego kod samostalne infekcije. Pasmine pasa s vise\u0107im u\u0161ima su sklonije nastanku upale vanjskog u\u0161nog kanala prouzro\u010dene gljivicama nego pasmine pasa s uspravnim u\u0161ima.<\/p>\n

Klju\u010dne rije\u010di:<\/strong> upala vanjskog u\u0161nog kanala, Malassezia spp., pasmine pasa<\/em><\/p><\/blockquote>\n","protected":false},"excerpt":{"rendered":"

N. Te\u0161in, I. Stan\u010di\u0107, Z. Kana\u010dki, J. Spasojevi\u0107, Z. Ru\u017ei\u0107, I. Gali\u0107 and Z. Kova\u010devi\u0107* Nade\u017eda TE\u0160IN, DVM, Junior Researcher,<\/p>\n","protected":false},"author":8,"featured_media":0,"menu_order":6,"comment_status":"closed","ping_status":"open","template":"","format":"standard","meta":{"footnotes":""},"categories":[21],"tags":[1839,949,1838],"issuem_issue":[1778],"ppma_author":[1831,1832,1833,1834,1835,1836,1837],"class_list":["post-6195","article","type-article","status-publish","format-standard","hentry","category-original-scientific-articles","tag-malassezia-spp","tag-pasmine-pasa","tag-upala-vanjskog-usnog-kanala","issuem_issue-veterinarska-stanica-54-6"],"yoast_head":"\nPrevalence and infestation degree of yeast in canine otitis externa - CROATIAN VETERINARY JOURNAL<\/title>\n<meta name=\"description\" content=\"Otitis externa is a persistent and recurrent problem, and a serious issue in veterinary medicine.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/journal.h3s.org\/?article=prevalence-and-infestation-degree-of-yeast-in-canine-otitis-externa\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Prevalence and infestation degree of yeast in canine otitis externa - 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